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. 2011 Dec 14;37(5):1234–1244. doi: 10.1038/npp.2011.311

Figure 3.

Figure 3

Blockade of extracellular signal-regulated kinase (ERK) phosphorylation inhibits memory retrieval. (a) Latency times measured during the retention trial were reduced by the administration of U0126 but not KN62. (b), Dose-dependent effects of U0126 on memory retrieval. Fifteen minutes before the retention trial, KN62 (1 μg/μl/side) or U0126 (0.25, 0.5, or 1 nmol/μl/side) were administered bilaterally into the hippocampus. Data are presented as the means±SEM (n=8–10 per group). *p<0.05, compared with the vehicle-treated group. (c, d), Immunoblotting (c) and quantitative analysis (d) of pCREB, pCaMKII, and pERK levels in hippocampal tissues after the administration of KN62 or U0126 before the retention trial. Fifteen minutes before the retention trial, KN62 (1 μg/μl/side) or U0126 (1 nmol/μl/side) were administered bilaterally into the hippocampus. The mice were immediately killed after the retention trial. Data are presented as the means±SEM (n=4 per group). *p<0.05, compared with the control group; #p<0.05, compared with the vehicle-treated group. Control, animals that did not undergo a retention trial; Vehicle, animals that underwent a retention trial but were treated only with vehicle; KN62, animals treated with KN62 that underwent a retention trial; U0126, animals treated with U0126 that underwent a retention trial.