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Production of 100 bp DNA ladder. (a) The pGEM-800 plasmid was partially digested by SmaI enzyme producing 8 fragments in length of 100–800 bp. (b, c) Electrophoresis of the prepared 100 bp DNA ladder. A 5 μL (lane 2) or 3 μL (lane 3) of DNA ladder prepared in the present study and 100 bp DNA marker from Takara (M) were submitted to 2% agarose (b) or 12% acrylamide (c) gel electrophoresis, respectively.
