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. 2011 Aug 15;31(9):1117–1129. doi: 10.1038/onc.2011.327

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Copyright © 2012 Macmillan Publishers Limited

This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Correlation between H-Ras expression level and DNA damage. (a) Dose-dependent stimulation of γ-H2A.X in Tet-on-regulated H-RasV12 HThy-ori cells treated with doxycycline for 48 h. (b) Increase in DNA fragmentation level upon H-RasV12 activation. Kinetics of double-strand breaks increase measured by pulse field gel electrophoresis in cells after doxycycline treatment (1 μg/ml). Schizosaccharomyces pombe chromosomes are used as DNA size markers. DNA fragmentation is analyzed on 1 million and 2 millions cells as indicated. Time-dependent stimulation of γ-H2A.X in Tet-on-regulated H-RasV12 HThy-ori cells treated with doxycycline (1 μg/ml) for indicated times. (c) Immunofluorescence of γ-H2AX and p22^phox after expression of H-RasV12. Magnification × 10 (middle panel) and × 100 (lower panel).