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. 2011 Aug 15;31(9):1117–1129. doi: 10.1038/onc.2011.327

Figure 7.

Figure 7

NADPH oxidase NOX4 is involved in H-RasV12-induced senescence. (a) Decrease in SAHF formation after NOX4 inactivation. Tet-on-regulated H-RasV12 HThy-ori cells were transduced with interference RNA against p22phox or NOX4 and treated or not with doxycycline (1 μg/ml) for indicated times, fixed and stained with DAPI (4',6-diamidino-2-phenylindole). Magnification × 63. Results were quantified by scoring 100 cells on three independent slides for each assay from two independent experiments. J3: sictrl vs sip22phox P=0.039, sictrl vs siNOX4 P=0.048; J6: sictrl vs sip22phox P=0.349; sictrl vs siNOX4 P=0.078 using Student's t-test. *P<0.05. Colocalizing HP1β/DAPI foci (b) and PML bodies (c) were quantified by scoring 100 cells from each slide. All experiments were realized with siRNA NOX4 pool from Dharmacon (Table 1).