Table 1.
Baboon Hormone Stimulation Protocols: Average Oocyte Stages at Collection and Expanded Blastocyst Rates
| [a] Protocol # | [b] # Days of 60 IU r-FSH | [c] # Days of 60 IU r-LH | [d] GnRH Agonist (9 days) | [e] hCG dose (IU) | [f] Collection Day | [g] # of Stim Trials1 | Average oocytes by stage at collection | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| [h] GV (Mean ± SD) | [i] GVBD Mean ± SD) | [j] PB1 (Mean ± SD) | [k] Total (Mean ± SD | [l] Avg # of Oocytes Fertilized (Mean ± SD)2 [% of Total] | [m] Ex Blasts Rate (%) | |||||||
| I | 9 | 0 | - | 1000 | 11 | 7 | 25.3 ± 18.9 | 5.4 ± 4.0 | 1.4 ± 1.6 | 32.7 ± 21.3 | 8.7 ± 7.6a [27] | 0/64 (0) |
| II | 9 | 3 | + | 1000 | 11 | 7 | 21.3 ± 12.9 | 3.7 ± 4.8 | 2.6 ± 2.6 | 29.4 ± 11.5 | 6.0 ± 6.5a (20) | 2/42 (5) |
| III | 9 | 0 | - | 2500 | 11 | 5 | 33.0 ± 33.0 | 9.6 ± 11.1 | 1.4 ± 1.1 | 48.6 ± 33.6 | 12.8 ± 12.3b [26] | 0/61 (0) |
| IV | 9 | 3 | - | 2500 | 11 | 20 | 32.8 ± 21.2 | 7.5 ± 7.7 | 8.2 ± 8.3 | 49.4 ± 30.2 | 16.9 ± 13.9b [34] | 2/338 (1) |
| V | 9 | 9 | - | 2500 | 11 | 16 | 24.3 ± 16.0 | 5.7 ± 5.1 | 4.4 ± 4.4 | 34.6 ± 20.9 | 11.6 ± 9.3b [34] | 11/185 (6) |
| VI | 9 | 9 | + | 5000 | 11 | 8 | 39.4 ± 31.6 | 12.5 ± 7.1 | 14.6 ± 7.4 | 66.7 ± 30.5 | 28.6 ± 13.4c (43) | 3/229 (1) |
| VII | 10-13 | 10-13 | - | 2500 | 12-15 | 15 | 28.0 ± 31.7 | 5.5 ± 5.9 | 8.4 ± 7.0 | 41.9 ± 28.9 | 13.7 ± 9.1b (33) | 3/205 (3) |
| VIII | 13 | 13 | - | 5000 | 15 | 7 | 27.6 ± 23.5 | 3.3 ± 5.0 | 10.4 ± 13.3 | 42.4 ± 36.4 | 14.9 ± 15.9b (35) | 1/104 (1) |
| VIX | 10-13 | 10-13 | + | 5000 | 12-15 | 11 | 20.7 ± 15.9 | 4.1 ± 4.1 | 9.2 ± 7.8 | 37.2 ± 22.7 | 13.5 ± 9.4b (36) | 0/148 (0) |
1
number of stimulation trial attempts
2
means followed by the same letter do not differ significantly from one another (p≤ 0.05)