Table 1.
Primer | Vector | Sequence (5′-3′) |
dbpA/BbssFW | pET-30 | GAC GAC GAC AAG GGA TTA AAA GGA GAA ACA AAA ATC |
dbpA/BbssREV | pET-30 | GAG GAG AAG CCC GGT TTA GTT ATT TTT GCA TTT TTC ATC |
dbpB/BbssFW | pET-30 | GAC GAC GAC AAG GGA TTA GTA GAA AGA ACA AAT GC |
dbpB/BbssREV | pET-30 | GAG GAG AAG CCC GGT TTA TTT CTT TTT TTT GCT TTT ATT AT |
dbpAB/BbssFW | pBSV2 | AATA GCA TGC ACA AGC CAG ATT GCA TAG C |
dbpAB/BbssREV | pBSV2 | AATA GGA TCC TTG ATT ATC GGG CGA AGA G |
dbpAB/BgFW | pBSV2 | AATA GCA TGC ACA TTA TTT GGC AAA CTG GC |
dbpAB/BgREV | pBSV2 | AATA GGA TCC GGT ACT TTA CGA CAG TCT TG |
dbpAB/BaFW | pBSV2 | AATA GCA TGC CCC CTG GCA AAA TAA AAT TC |
dbpAB/BaREV | pBSV2 | AATA GGA TCC TTA TTT TTG ATT TTT AGT TTG TTT TTC |
NOTE. Restriction enzyme sites in the primers are bold type.