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. Author manuscript; available in PMC: 2013 Feb 21.
Published in final edited form as: Anal Chem. 2012 Feb 7;84(4):1941–1947. doi: 10.1021/ac300070t

Figure 5.

Figure 5

(a) Kinetic responses of spectral peak position during the immobilization of protective antigen (PA). (b) Schematic of scFv binding to PA immobilized on the Au nanohole surface via amine coupling. (c) Binding kinetics curves of 14B7 scFv antibody to PA with various concentrations. (d) Binding kinetic curves of 100 nM scFv antibodies to PA. These scFv molecules having the same molecular weight, but different dissociation constants with the PA in the range of around 10 pM to 100 nM are sequentially injected. Small noise peaks just prior to dissociation event are likely caused by the operation of the four-way mechanical valve, but they do not affect the calculation of binding kinetics.