Table 1.
Summary of kinetic values for 14B7 scFv family measured by the nanohole SPR system. The kinetic parameters (kd, ka, and Kd) are calculated from kinetic curves shown in Figure 5. The error terms in kd and ka are from variations in curve fitting. The mass action numbers (Ma#), defined as Kd/LOD concentration, are calculated based on the spectral response to a concentration assay shown in Figure 6.
|
kd (× 10−4 s−1) |
ka (× 105 M−1 s−1) |
Kd (nM) |
LOD (nM) |
Ma# | |
|---|---|---|---|---|---|
| L97A | 101 ± 30 | 2.76 ± 0.43 | 36.7 | 20 | 1.9 |
| 14B7 | 34 ± 0.7 | 2.92 ± 0.23 | 11.7 | 10 | 1.1 |
| 1H | 1.6 ± 0.1 | 5.47 ± 0.37 | 0.29 | 1 | 0.3 |
| M18 | 1.1 ± 0.1 | 5.97 ± 0.38 | 0.19 | 1 | 0.2 |