FIGURE 3.

Amidoxime reductase activity is regulated during differentiation of preadipocytes into mature adipocytes. A, benzamidoxime reductase activity measured in the mitochondria and the 6,500 × g supernatant containing both the microsomes and cytosol isolated from preadipocytes (0 days) and 2- and 8-day differentiated adipocytes. Incubations were performed with 20 μm benzamidoxime in cells at 0, 2, and 8 days after differentiation start for 30, 20, and 10 min, using 30, 25, and 10 μg of mitochondria or 6,500 × g supernatant proteins, respectively. Incubations were performed in the absence (gray bars) and in the presence (white bars) of KCN. Results are presented as mean ± S.D. (n = 3). ***, p < 0.001. B, protein expression levels of MOSC2, CYB5A, CYB5B, and CYB5R3 in the cell lysates isolated from preadipocytes (pre) (0 days) and 8-day differentiated adipocytes. Also shown are the levels for SCD1 (stearoyl-CoA desaturase 1), a marker for the adipocyte differentiation and calnexin as a loading control.