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Tyr-192 is the major chlorination target, whereas Tyr-18 is the major nitration target in HDL isolated from human atherosclerotic lesions. HDL was isolated by ultracentrifugation from atherosclerotic lesions of carotid arteries harvested from humans (n = 8). Regiospecific oxidation of apoA-I was determined in tryptic and Glu-C digests of HDL, as described in the legends to Fig. 4. Results are representative of those from two independent experiments.
