FIGURE 6.
TGF-β activity is decreased by a synthetic peptide derived from the LRR6 internal region of decorin (Dcn). A, wild type and decorin-deficient myoblasts were transiently transfected with plasmids containing p3TP-lux and pRL-SV40 sequences and incubated with 75 nm hDcnHA in the absence or presence of 225 nm synthetic peptides from the LRR6 region of decorin, LRR6 (Full), LRR6i (In), or LRR6e (Ext). After 6 h of TGF-β1 (1.0 ng/ml) treatment, cells were lysed and reporter activities were determined. Values correspond to the mean ± S.D. from three independent experiments (*, #, and &, p < 0.05). B, wild type and decorin-deficient myoblasts were transiently transfected with p3TP-lux and pRL-SV40 plasmids and incubated with 75 nm hDcnHA-Full in the absence or presence of different amounts of LRR6 (Full, In, or Ext) or scramble peptide (Scramble). Treatment with TGF-β1 (1.0 ng/ml) and reporter activities were determined as in A. Values correspond to the mean ± S.D. from three independent experiments (*, p < 0.05; # and &, p < 0.001). C, decorin-deficient myoblasts were incubated with TGF-β1 (5.0 ng/ml) plus 75 nm hDcnHA in the absence or presence of 225 nm synthetic peptides LRR6: Full, In, or Ext, or scramble (Scr) peptide. Total RNA was isolated and CTGF and GAPDH expression was determined by qRT-PCR according to “Experimental Procedures.” Values correspond to the mean of dCT value ± S.D. of three independent experiments (* and #, p < 0.05).