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. 2011 Dec 29;287(9):6421–6430. doi: 10.1074/jbc.M111.310342

FIGURE 2.

FIGURE 2.

GLP-1 regulates adipogenesis via GLP-1R. A and B, expression of Glp-1R mRNA and protein in 3T3-L1 cells was measured at different time points during adipogenesis (n = 8). C, 3T3-L1 pre-adipocytes were infected with GLP-1R shRNA expressing lentivirus (shRNA2 and shRNA3) or scrambled shRNA control. Glp-1R expression was analyzed by qPCR (n = 4). D, quantitative analysis of adipocyte differentiation using high-throughput image analysis in cells infected with GLP-1R shRNA expressing lentivirus or scrambled shRNA control (n = 12) and treated with GLP-1. E–G, analysis of PPARγ, A-Fabp, C/ebpβ/δ mRNA and protein expression in 3T3-L1 pre-adipocytes infected with GLP-1R shRNA-expressing lentivirus (shRNA2 and shRNA3) or scrambled shRNA and treated with GLP-1 (n = 4). Cells were analyzed at indicated time points post induction of differentiation. γ-tubulin was used as a loading control. The graphs represent mean ± S.E. (**, p < 0.01, ***, p < 0.001).