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. 2012 Jan 4;287(9):6912–6927. doi: 10.1074/jbc.M111.294025

FIGURE 5.

FIGURE 5.

Tannic acid inhibits BACE1 protein at the post-transcriptional level in PSAPP mice. A, Western blots are shown using an amino (N)-terminal APP polyclonal antibody (pAb N-APP; holo-APP is shown), a carboxyl-terminal human sAPP-α monoclonal antibody (mAb 2B3; sAPP-α is shown), a carboxyl-terminal human sAPP-β monoclonal antibody (mAb 6A1; sAPP-β is shown), and a carboxyl-terminal β-site APP cleaving enzyme 1 polyclonal antibody (pAb BACE1). Actin is shown as a loading control for each blot. Densitometry analyses are shown for the ratio of: B, sAPP-α to actin; C, sAPP-β to actin; and D, BACE1 to actin. E, QPCR data reveal no differences between groups on BACE1 expression. Data are expressed as relative fold over WT-V mice. Data for A–D were obtained from PSAPP mice treated with vehicle (PSAPP-V, n = 16) or with TA (PSAPP-TA, n = 16) for 6 months beginning at 6 months of age. Wild-type mice treated with vehicle (WT-V, n = 16) or with TA (WT-TA, n = 16) were also included for E. The statistical comparisons for C and D are between PSAPP-V and PSAPP-TA mice.