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Kinetic analysis
| Class II activitya |
Class I activity |
|||
|---|---|---|---|---|
| Km | kcat | Km | kcat | |
| μm | s−1 | μm | s−1 | |
| WT | 0.5 ± 0.1 | 1.1 ± 0.1 | 2.0 ± 0.1 | 2.6 ± 0.1 |
| R356A | 6 ± 3 | 1.1 × 10−3 | 0.7 ± 0.1 | 1.1 ± 0.6 |
| N451A | 6 ± 4 | 0.016 | 1.4 ± 0.3 | 1.9 ± 0.1 |
a Determined in D621A background to abrogate class I activity.
