FIGURE 1.

hGLuc-based PCA strategy for studying protein-protein interactions of Tau. A, schematic presentation of hGLuc fragment-tagged reporter constructs used in this study for live cell analysis of protein-protein interactions of Tau. 0N4R isoform of human Tau was used throughout the study. B, immunofluorescence micrograph of N2a cell coexpressing Tau-GLuc2 and EGFP-tubulin. The nuclei were counter-stained with Hoechst 33342. C, Western blot analysis of expression of GLuc fragment-tagged reporter constructs in N2a cells. The blots were stained with antibodies to Tau (Tau-5), Pin1, and GAPDH as a loading control.