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. 2012 Jan 10;287(9):6743–6752. doi: 10.1074/jbc.M111.309385

FIGURE 4.

FIGURE 4.

GABAA receptor activation promotes Tau phosphorylation at the AT8 epitope in mature cortical neurons. A, primary rat cortical neurons (21 DIV) were treated with indicated concentrations of butethal and desalkylflurazepam for 6 h. As a control, the neurons were treated with vehicle only. Cell extracts were analyzed on Western blots with AT8, TG3, and PHF13 phospho-specific Tau antibodies. GAPDH antibody was used as a loading control. B, optical density quantification of Tau phosphorylation at the AT8 epitope from the Western blot (Fig. 4A). C, primary rat cortical neurons (21 DIV) were treated with 50 μm butethal (BUT) and desalkylflurazepam (DAF) for 48 h or treated for 24 followed by a 24-h washout period. As a control, the neurons were treated with vehicle only. The samples were analyzed as in Fig. 4A. Veh, vehicle.