FIGURE 3.

Calculated difference transient spectra of the photo-oxidized β₁(190) heme at +2 ps (solid line) obtained by subtracting the raw transient spectra of sGC (dashed line), containing only the contribution of NO photo-dissociation, from the raw spectra of photo-excited β₁(190)-NO (dotted line) containing both the contributions of NO photo-dissociation and photo-oxidation. The raw spectra were normalized at the minimum of the bleaching (396 nm). This calculated spectrum is remarkably similar to that of unliganded of β₁(190) (Fig. 2C) and to that of SVD1 components for β₁-NO (supplemental Fig. S1C).