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TUG colocalizes with ERGIC-53 by confocal microscopy. A, endogenous TUG and ERGIC-53 were detected in HeLa cells using indirect immunofluorescence and confocal microscopy. Cells were untreated or treated with 30 μm nocodazole or 10 μg/ml brefeldin A for 2 h prior to staining, as indicated. B and C, endogenous TUG and Vti1a (B) and TUG and GM130 (C) were imaged as in A. The insets show enlargements of the perinuclear region of the merged images. Scale bars, 10 μm.
