FIGURE 7.

siRNA-mediated TUG depletion has no large effect on ubiquitylated protein abundance or p97 distribution. A, HeLa were transfected using control (scrambled) siRNA, TUG siRNA A, or TUG siRNA B, as indicated, and were lysed 48 h after transfection. Proteins were separated by SDS-PAGE and immunoblotted as indicated. Hsp90 and transferrin receptor were used as loading controls. B, endogenous TUG and p97 were imaged in HeLa cells using indirect immunofluorescence and confocal microscopy. Cells were treated with the indicated siRNAs 48 h prior to fixation. Scale bar, 20 μm.