TABLE 2.
Reaction conditions | Enzyme |
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---|---|---|---|---|---|---|---|---|
NpAS |
DgAS |
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30 °C |
37 °C (optimum temperature) |
30 °C |
50 °C (optimum temperature) |
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Sucrose 100 mm | Sucrose, 100 mm, fructose, 100 mm | Sucrose, 100 mm | Sucrose, 100 mm, fructose, 100 mm | Sucrose, 100 mm | Sucrose, 100 mm, fructose, 100 mm | Sucrose, 100 mm | Sucrose, 100 mm, fructose, 100 mm | |
% of glucosyl residues in turanose | 20.6 | 51.9 | 19.4 | 44.0 | 12.9 | 34.1 | 12.4 | 33.4 |
% of glucosyl residues in trehalulose | 4.5 | 11.9 | 4.1 | 9.0 | 12.8 | 35.2 | 10.6 | 26.5 |