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. 2011 Dec 30;287(9):6642–6654. doi: 10.1074/jbc.M111.322917

TABLE 2.

Comparison of turanose and trehalulose formation by DgAS and NpAS expressed as the percentage of the glucosyl units coming from sucrose transferred onto fructose

Reaction conditions Enzyme
NpAS
DgAS
30 °C
37 °C (optimum temperature)
30 °C
50 °C (optimum temperature)
Sucrose 100 mm Sucrose, 100 mm, fructose, 100 mm Sucrose, 100 mm Sucrose, 100 mm, fructose, 100 mm Sucrose, 100 mm Sucrose, 100 mm, fructose, 100 mm Sucrose, 100 mm Sucrose, 100 mm, fructose, 100 mm
% of glucosyl residues in turanose 20.6 51.9 19.4 44.0 12.9 34.1 12.4 33.4
% of glucosyl residues in trehalulose 4.5 11.9 4.1 9.0 12.8 35.2 10.6 26.5