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. 2012 Jan 10;287(9):6350–6361. doi: 10.1074/jbc.M111.299081

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — α-NRXN protein is present in β cells. A, serial sections of human, rat, and mouse pancreas were stained for insulin (top) and NRXN (bottom). Omission of primary antibody in parallel control experiments abolished the observed islet staining (data not shown). B, cytosolic and plasma membrane fractions (10 μg each, lanes 1–2) of INS-1E cells as well as total lysate (30 μg, lane 3) were immunoblotted with a pan-NRXN antibody. Protein immunoprecipitated from INS-1E cell extracts with a NRXN1 antibody (NRXN IP, lane 5) or an isotype control antibody (Control IP, lane 4) was analyzed on the same blot. Lysates of control COS-7 cells (Control) and COS-7 cells overexpressing CFP or CFP-tagged NRXN1α and NRXN2α (lanes 6–9) were immunoblotted in parallel to further validate the pan-NRXN antibody. N.S. indicates a nonspecific band detected by the pan-NRXN antibody in the lysate (lane 3) but not in anti-NRXN1 immunoprecipitates (lane 5).