FIGURE 2.

Quantification of NRXN transcripts in β cells by absolute RT-qPCR. NRXN mRNA transcript numbers in 50 ng of starting RNA from A, rat islets, the rat β-cell lines INS-1E and INS-1 832/13, and rat brain as well as B, human islets and C, mouse islets were quantified by absolute qPCR using a set of standard curves for each reaction with known NRXN copy numbers. Rat islet data are also represented as an inset in panel A. Data are represented as mean ± S.E. from three individual tissue, islet or culture preparations assayed in triplicate.