FIGURE 5.

Interaction of αCT-αCT reduced and alkylated derivatives with human α7nAChR heterologously expressed in Xenopus oocytes. To evaluate effects of the toxin, cells were challenged at a regular interval (2 min) with brief ACh test pulses (200 μm, 5 s). Following a stabilization period, toxin was applied at 1 μm for at least 1 min. The response to ACh (200 μm) was tested again, and recovery from inhibition was monitored over several minutes. Results obtained with αCT-αCT-2AE are illustrated in A and C, whereas effects of αCT-CT-4AE are shown in B and D. Average effects of αCT-αCT-2AE measured on four cells with 10-min recovery are presented in C. Average effects of αCT-αCT-4AE on four cells are presented in D. Bars indicate S.E.