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. 2011 Dec 28;287(9):6208–6217. doi: 10.1074/jbc.M111.310375

FIGURE 2.

FIGURE 2.

Effects of Notch activation on LPS-triggered IL-6, TNF-α, and IL-10 production. A, mouse primary peritoneal macrophages (5 × 105) were transiently transfected with NICD1 or NICD2 or mock vector for 48 h and then were stimulated with 100 ng/ml LPS or 0. 33 μm CpG-ODN for 8 h. IL-6, TNF-α, and IL-10 in the supernatants were measured by ELISA. B, mouse primary peritoneal macrophages were transiently transfected with NICD1, N1-6MT, or mock vector for 48 h, and then were stimulated with 100 ng/ml LPS for 8 h. IL-6, TNF-α, and IL-10 in the supernatants were measured by ELISA. Data are shown as mean ± S.D. (error bars; n = 3) of one representative experiment. Similar results were obtained in three independent experiments. *, p < 0.05; **, p < 0.01.