FIGURE 5.

Notch signaling inhibits TLR-triggered NF-κB transcription activity via MyD88/TRAF6 and TRIF pathway. HEK293T cells were transfected with 40 ng of NF-κB reporter gene plasmid, 10 ng of pTK-Renilla-luciferase, and 40 ng of MyD88 (A), TRAF6 (B), or TRIF (C) expressing plasmid alone or with 160 ng of NICD1 or NICD2 expressing plasmid. After 24 h, NF-κB reporter activities in lysates were measured by luciferase assay. Empty control vector was added to each sample to ensure transfection of the equal amount of DNA. Luciferase activity is normalized to Renilla luciferase activity and is presented relative to basal luciferase activity. Data are the mean ± S.D. (error bars) of five samples in one experiment representative of similar results obtained in three independent experiments. **, p < 0.01.