Skip to main content
. 2011 Dec 28;287(9):6208–6217. doi: 10.1074/jbc.M111.310375

FIGURE 6.

FIGURE 6.

Notch-mediated ERK1/2 inactivation is responsible for the inhibition of NF-κB transcription activity. A, RAW264.7 cells were transfected with 200 ng of NF-κB luciferase reporter plasmids, 20 ng of pTK-Renilla luciferase reporter plasmids. After 6 h, cells were pretreated with 20 nm PD98059 for 30 min and then pretreated with 2 μm GSI or dimethyl sulfoxide (DMSO). After 24 h, the cells were stimulated with 100 ng/ml LPS for 6 h. Luciferase activity was measured. Data are shown as mean ± S.D. (error bars; n = 5) of one representative result from three independent experiments with similar results. *, p < 0.05; **, p < 0.01. B, RAW264.7 cells were transfected with 5 nm siERK1 and siERK2 or control siRNA (si-Non) for 24 h. The cells were transfected with 200 ng of NF-κB luciferase reporter plasmids, 20 ng of pTK-Renilla luciferase reporter plasmids. After 6 h, cells were pretreated by 2 μm GSI or dimethyl sulfoxide. After 24 h, the cells were stimulated with 100 ng/ml LPS for 6 h. Luciferase activity was measured. Data are shown as mean ± S.D. (error bars; n = 5) of one representative result from three independent experiments with similar results. *, p < 0.05; **, p < 0.01.