Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Dec 19;287(9):6441–6453. doi: 10.1074/jbc.M111.322024

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 2. — Expression of PST H331K, PST140, and sPST140 inhibits NCAM polysialylation by endogenous PST in SW2 cells. Myc-tagged PST, sPST, PST H331K, PST140, and sPST140 were transiently expressed in SW2 small cell lung carcinoma cells containing endogenous PST and polysialylated NCAM. Eighteen hours post-transfection, cells were fixed and indirect immunofluorescence was performed using the anti-Myc antibody to analyze enzyme localization (Myc, Localization) or the OL.28 anti-polySia antibody to analyze total polysialylation (OL.28, Polysialylation). Overlaying the fluorescent signals for PST protein localization and polysialylation demonstrated the impact that expressing exogenous PST proteins had on the polysialylation of endogenous SW2 cell NCAM (Myc/OL.28, Merge). Cells expressing exogenous PST proteins are marked with yellow arrowheads. Cells were examined using a Zeiss 200M inverted confocal microscope, ×63 oil immersion objective. Scale bar = 10 μm.