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. 2011 Dec 19;287(9):6441–6453. doi: 10.1074/jbc.M111.322024

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — NCAM polysialylation by endogenous PST is inhibited by expression of PST127 but not PST71 or PST62. Myc-tagged PST140, PST127, PST71, and PST62 were transiently expressed in SW2 small cell lung carcinoma cells. Eighteen hours post-transfection, cells were fixed and indirect immunofluorescence was performed to analyze enzyme localization (Myc, Localization) or total polysialylation (OL.28, Polysialylation). Overlaying the fluorescent signals for PST protein localization and polysialylation demonstrated the impact that expressing exogenous PST proteins had on the polysialylation of endogenous SW2 cell NCAM (Myc/OL.28, Merge). Cells expressing exogenous PST proteins are marked with yellow arrowheads. Cells were examined using a Zeiss 200M inverted confocal microscope, ×63 oil immersion objective. Scale bar = 10 μm.