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. 2012 Jan 4;287(9):6628–6641. doi: 10.1074/jbc.M111.291179

FIGURE 6.

FIGURE 6.

Mitochondrial F1F0-ATP synthase (Complex V) assembly is affected by DmGSTO1 expression in park1 mutants. A and B, mitochondrial protein extracts from the thorax of mutant fly lines were subjected to blue native PAGE, followed by Western blot analysis with anti-ATP synthase α subunit antibody. Three bands were detected: supercomplex (>800 kDa), assembled ATP synthase (Complex V, >600 kDa), and F1 subcomplex (>400 kDa). Prohibitin was used as a mitochondrial loading control. A, the assembly of ATP synthase (Complex V) was significantly decreased in park1 mutants. The amount of assembled ATP synthase was restored by DmGSTO1A up-regulation in park1 mutants. Error bars, S.D. The experimental significance was determined by one-way ANOVA (**, p < 0.01; *, p < 0.0001). Experiments were performed in quintuplicate. B, The amount of assembled ATP synthase tended to decrease more in park1/DmGSTO1null double mutants than park1 mutants; however, the effect was not statistically significant. Error bars, S.D. The experimental significance was determined by one-way ANOVA (**, p < 0.0001; *, p < 0.001; ns, not significant). Experiments were performed in quadruplicate.