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. 2011 Dec 29;287(9):6326–6335. doi: 10.1074/jbc.M111.246553

FIGURE 4.

FIGURE 4.

PMA and Go6983 are active in the juvenile rat slice preparation at the concentrations used in previous slice experiments. A, representative raw data show that application of 1 μm PMA to rat cerebella slices leads to an increase in mIPSC amplitude (top left, control mIPSC frequency; top right, mIPSC frequency after exposure to PMA for 11 min; data were obtained from one cell). This increase in mIPSC frequency can be inhibited by pretreatment of slices with 1 μm Go6983 (Go) (bottom left) mIPSC frequency after exposure of slices to 1 μm Go6983 for 40 min; bottom right, mIPSC frequency after additional exposure to 1 μm PMA for 11 min; data were obtained from one cell). Top and bottom traces were recorded from two different Purkinje cells. Experiments were carried out in the absence of bicuculline. B, aggregate data show that under control (ctl) conditions application of 1 μm PMA leads to a significant increase in mIPSC frequency (n = 4; p = 0.016, paired Student's t test), whereas application of 1 μm PMA after preincubation to 1 μm Go6983 (Go) prevents an increase in mIPSC frequency (p = 0.868 for mIPSC frequency in Go6983 compared with mIPSC frequency in Go693 and PMA, paired Student's t test).