FIGURE 4.

Snail, but not Slug, increases pancreatic cancer cell collagen I binding, collagen I-driven motility, and α2β1-integrin expression. A and B, stable AsPC1 cells were induced with doxycycline (2 μg/ml) for 24 h and then plated onto tissue culture plates coated with thin-layer collagen, allowed to adhere at 37 °C for 10 min, washed, and then stained. Adherent cells were photographed (A) and counted (B). C and D, AsPC1 cells were induced with doxycycline (2 μg/ml) for 24 h, plated onto thin-layer type I collagen matrix overlaid with colloidal gold, and allowed to migrate for 24 h, and the tracks were photographed (C) and quantified (D). E, equal numbers of AsPC1 cells were induced with doxycycline for 24 h, surface-labeled with biotin, and lysed, and cell lysates were immunoprecipitated with streptavidin and analyzed for α2-, α3-, and β1-integrin and transferrin receptor (loading control) by Western blotting. Error bars in B and D indicate S.E. The results are representative of at least three independent experiments. *, p < 0.05.