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. 2012 Jan 6;287(9):6284–6295. doi: 10.1074/jbc.M111.293001

FIGURE 9.

FIGURE 9.

Flufenamic acid sensitizes S49 (OS 4–15) cells to intrinsically induced apoptosis. A, S49 (OS 4–15) cells were treated with 250 mOsm mannitol for 6 h in the presence and absence of 200 μm FFA and examined for the loss of membrane integrity. The addition FFA to inhibit the RVI response sensitized S49 (OS 4–15) cells to undergo cell death. B, S49 (OS 4–15) cells were treated with 0.5 μm staurosporine, 10 μm thapsigargin, or 75 mJ/cm2 UV for 10 h in the presence and absence of 220 μm FFA and examined for the loss of membrane integrity (top) and degraded DNA (bottom) using flow cytometry. Again, the presence of FFA sensitized the S49 (OS 4–15) cells to undergo apoptosis. Data represent the mean (± S.E.) of three independent experiments. *, p < 0.001 versus control.