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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Pflugers Arch. 2011 Dec 8;463(4):537–548. doi: 10.1007/s00424-011-1061-z

Figure 7.

Figure 7

Western blots of Kv4.3. Molecular weight markers are indicated in kDa. (A) Kv4.3 was detected at 65 kDa in rat brain (rB) and rabbit brain (RB) using polyclonal α-Kv4.3 primary antibody (1°Ab) (left section of membrane), and was markedly reduced when treated with 1°Ab pre-incubated with antigen, Ag (right). (B) Kv4.3 was also detected at the same molecular weight using a monoclonal 1°Ab in both rodent brain and human atrium (HA), but was markedly reduced in negative control rabbit lung (RL), and absent with secondary antibody (2°Ab) only. (C) Increasing protein concentrations (5-10 μg) of whole human atrial homogenate from two β-blocked (BBYes) and two non-β-blocked (BBNo) patients treated with monoclonal α-Kv4.3 (upper section) or monoclonal α-GAPDH (lower section).