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. 2012 Jan 4;7(3):464–469. doi: 10.1021/cb2004252

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Copyright © 2012 U.S. Government

This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org.

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Live cell imaging of HEK293A cells stably expressing SNAP-β2ADR or SNAP-NK1R transfected with β-arrestin2-TagRFP. From Supplementary Videos 3 and 4. (a) Images show the redistribution of β-arrestin2-TagRFP from the cytosol to the plasma membrane in the presence of isoproterenol in cells expressing SNAP-β2ADR. Boxes highlight the redistribution to the cell surface in adjacent cells. (b) Comparison of the colocalization of SNAP-β2ADR or SNAP-NK1R with β-arrestin2-TagRFP after agonist-induced uptake (isoproterenol for SNAP-β2ADR or Substance P for SNAP-NK1R) and 5 mM TCEP treatment. β-Arrestin2-TagRFP colocalizes with SNAP-NK1R labeled endosomes but not with SNAP-β2ADR. Scale bar, 10 μm.