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. 2012 Jan 27;94(1):20–29. doi: 10.1093/cvr/cvs026

Figure 3.

Figure 3

Quantification of aortic superoxide production in Nox2-Tg ApoE−/− and ApoE−/− mice. Quantification of NADPH-dependent thoracic aortic superoxide production by (A) 2-hydroxyethidium fluorescence HPLC showed that superoxide production was significantly increased in aortas from 24-week-old Nox2-Tg ApoE−/− mice vs. ApoE−/− mice (*P< 0.05, n= 9–13). Data are presented as box plots of the tiron-inhibitable fraction of 2-hydroxyethidium production. (B) Lucigenin-enhanced chemiluminescence showed that superoxide production was significantly increased in aortas from 9 to 18-week-old Nox2-Tg ApoE−/− mice vs. ApoE−/− mice (*P< 0.05, n= 13–17). (C) 2-Hydroxyethidium fluorescence in frozen sections of the aortic root from 9-week-old Nox2-Tg ApoE−/− mice vs. ApoE−/− mice demonstrated that endothelial superoxide production was significantly increased in aortic roots from 9-week-old Nox2-Tg ApoE−/− mice vs. ApoE−/− mice (*P< 0.05, n= 8–5). Data are presented as box plots. Area of 2-hydroxyethidium fluorescence in frozen sections was normalized to vessel length.