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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Dev Biol. 2011 Dec 20;363(1):40–51. doi: 10.1016/j.ydbio.2011.12.021

Figure 10.

Figure 10

SAS1B mediated inhibition of mouse in vitro fertilization. (A) Capacitated mouse sperm were incubated with varying concentrations of rSAS1B prior to fertilization of cumulus intact oocytes. The percentage of fertilized eggs decreased with increased concentration of SAS1B compared to a controls including the oocyte cytoplasmic protein recombinant Ecat1 or PBS without proteins. (B) Inhibition of fertilization of zona-intact eggs in presence of SAS1B antibody (black bar) compared to pre-immune (white bar) controls. Bars represent mean ± SEM; numbers, eggs per group. Significant P-value differences from no protein (PBS), recombinant Ecat1 (50 μg/ml) or preimmune controls were marked with asterisk (*, P ≤ 0.02 to 0.008).