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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Dev Biol. 2011 Dec 20;363(1):40–51. doi: 10.1016/j.ydbio.2011.12.021

Figure 2.

Figure 2

Localization of SAS1B in developing ovary by IHC and in M2 oocytes by IF. (A) SAS1B expressed in ooplasm of secondary (S) follicles beginning in day 3 ovary (A2). No SAS1B was detected in naked oocytes (A1), primordial (M) and primary (P) follicles (A5). Panels 1, 2, 3, 4, 5 and 6 represent postpartum ovaries at day 0, 3, 7, 14, 56 and 14 respectively. Preimmune control, A6. (B) In unpermeablized zona-intact ovulated M2 oocytes, SAS1B was concentrated in a dome shaped microvillar domain on the surface of the oocyte plasma membrane. Eccentric nuclei (blue) were antipodal to the SAS1B positive domain. Preimmune control panels: 4, 5 and 6. Panels: 1 and 4, phase; 2 and 5, fluorescence; 3 and 6, merged image of DAPI/fluorescence.