Interaction affinity between SAS1B and SLLP1. Binding curves were observed when six different analyte concentrations of SAS1B (400 - top, 300, 200, 100, 50, 25 and 0 nM - bottom) were injected (arrow) for 3 min over immobilized SLLP1 at a flow rate of 30 μl/min in running buffer using Biacore 3000 system. Following the binding phase, the sensograms showed a very low dissociation of SAS1B from the chip.