Table 2.
Adipose tissue gene expression levels in CB1+/+ and CB1-/- mice fed chow, a HF or a HF/FO diet during 6 weeks
| chow | HF | HF/FO | ||||
|---|---|---|---|---|---|---|
| CB1+/+ | CB1-/- | CB1+/+ | CB1-/- | CB1+/+ | CB1-/- | |
| Endocannabinoid system | ||||||
| CB1 (Cnr1) | 1.0 ± 0.1 | ND | 1.0 ± 0.1 | ND | 1.1 ± 0.2 | ND |
| Napepld | 1.0 ± 0.1 | 0.9 ± 0.1 | 1.4 ± 0.1# | 1.3 ± 0.2# | 1.2 ± 0.1 | 1.1 ± 0.1 |
| Faah | 1.0 ± 0.3 | 1.8 ± 0.5 | 1.1 ± 0.6 | 1.1 ± 0.4 | 1.0 ± 0.5 | 1.3 ± 0.6 |
| Adipocyte proliferation and differentiation | ||||||
| Pparγ2 (Pparg) | 1.0 ± 0.2 | 1.0 ± 0.1 | 1.4 ± 0.1 | 1.2 ± 0.3 | 1.0 ± 0.1$ | 0.9 ± 0.1 |
| C/ebpα (Cebpa) | 1.0 ± 0.1 | 1.0 ± 0.1 | 1.2 ± 0.0 | 1.3 ± 0.3 | 1.0 ± 0.1 | 0.9 ± 0.1 |
| Ap2 (Fabp4) | 1.0 ± 0.1 | 0.9 ± 0.1 | 1.5 ± 0.0# | 1.2 ± 0.2 | 1.4 ± 0.1 | 1.2 ± 0.1 |
| Adiponectin (Adipoq) | 1.0 ± 0.2 | 0.9 ± 0.2 | 1.3 ± 0.1 | 1.3 ± 0.4 | 1.3 ± 0.1 | 1.0 ± 0.1 |
| Trigyceride synthesis | ||||||
| Srebp-1c (Srebf1) | 1.0 ± 0.1 | 1.2 ± 0.1 | 1.5 ± 0.1# | 1.8 ± 0.4 | 1.1 ± 0.0$ | 1.1 ± 0.0 |
| Acc1 (Acaca) | 1.0 ± 0.2 | 1.1 ± 0.3 | 0.9 ± 0.1 | 0.9 ± 0.3 | 0.6 ± 0.1$ | 0.5 ± 0.1# |
| Fas (Fasn) | 1.0 ± 0.2 | 1.1 ± 0.2 | 1.1 ± 0.1 | 1.0 ± 0.3 | 0.7 ± 0.1$ | 0.4 ± 0.0# |
| Scd1 | 1.0 ± 0.1 | 0.9 ± 0.1 | 2.8 ± 0.1# | 3.1 ± 0.7# | 0.9 ± 0.1$ | 1.1 ± 0.1$ |
| Pepck (Pck1) | 1.0 ± 0.2 | 0.9 ± 0.1 | 1.0 ± 0.1 | 1.3 ± 0.4 | 1.1 ± 0.1 | 0.9 ± 0.1 |
| Fatty acid uptake and transport | ||||||
| Cd36 | 1.0 ± 0.1 | 1.1 ± 0.1 | 1.1 ± 0.0 | 1.2 ± 0.1 | 1.4 ± 0.2 | 1.1 ± 0.1 |
| Fatp4 (Slc27a4) | 1.0 ± 0.1 | 1.0 ± 0.1 | 1.2 ± 0.0 | 1.4 ± 0.2 | 1.2 ± 0.1 | 1.1 ± 0.1 |
| Fatty acid hydrolysis | ||||||
| Lpl | 1.0 ± 0.3 | 0.9 ± 0.2 | 1.2 ± 0.1 | 0.9 ± 0.2 | 1.4 ± 0.2 | 1.0 ± 0.1 |
| Gpihbp1 | 1.0 ± 0.1 | 1.2 ± 0.1 | 1.2 ± 0.1 | 1.2 ± 0.1 | 1.3 ± 0.1 | 0.9 ± 0.0 |
| Angptl3 | 1.0 ± 0.1 | 0.9 ± 0.1 | 1.2 ± 0.1 | 1.1 ± 0.1 | 1.0 ± 0.1 | 1.0 ± 0.0 |
| Angptl4 | 1.0 ± 0.1 | 1.0 ± 0.1 | 1.3 ± 0.1 | 1.2 ± 0.1 | 1.5 ± 0.1 | 1.1 ± 0.1 |
| Apoc1 | 1.0 ± 0.2 | 1.2 ± 0.1 | 1.1 ± 0.0 | 1.1 ± 0.0 | 0.7 ± 0.1$ | 0.9 ± 0.1 |
| Apoc3 | 1.0 ± 0.2 | 0.7 ± 0.1 | 0.6 ± 0.1 | 0.4 ± 0.0 | 0.2 ± 0.0$ | 0.2 ± 0.0# |
| Macrophage infiltration | ||||||
| Cd68 | 1.0 ± 0.1 | 1.0 ± 0.1 | 1.2 ± 0.0 | 1.0 ± 0.1 | 1.3 ± 0.1 | 1.0 ± 0.1 |
| Adipocyte lipolysis | ||||||
| Hsl (Lipe) | 1.0 ± 0.2 | 1.1 ± 0.2 | 1.2 ± 0.1 | 1.0 ± 0.2 | 1.2 ± 0.1 | 1.1 ± 0.1 |
| Atgl (Pnpla2) | 1.0 ± 0.2 | 1.1 ± 0.2 | 1.5 ± 0.2 | 1.4 ± 0.4 | 1.2 ± 0.1 | 1.2 ± 0.2 |
| Fatty acid oxidation | ||||||
| Cpt1a | 1.0 ± 0.1 | 1.2 ± 0.2 | 1.6 ± 0.2# | 1.2 ± 0.2 | 1.3 ± 0.1# | 1.1 ± 0.1 |
Expression levels were normalized to cyclophilin expression and values are given as means ± SEM for n = 4-8; # p < 0.05 compared to chow group of the same genotype, $ p < 0.05 compared to HF group of the same genotype (Student t-test). ND, non-detectable.
General linear model analysis revealed overall effects for the following parameters (p < 0.05):
Chow versus HF: aP2, Scd1, Srebp-1c.
Chow versus HF/FO: Fas.
HF versus HF/FO: Fas, Scd1, Srebp-1c.