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. Author manuscript; available in PMC: 2012 Mar 19.
Published in final edited form as: Eur J Neurosci. 2006 May;23(9):2297–2310. doi: 10.1111/j.1460-9568.2006.04734.x

Fig. 8.

Fig. 8

EFS-induced pCREB signaling supported by DHPR recombinant CaV1.2 and CaV1.3a L-type Ca2+ channels in hippocampal neurons. (A) The diagrams of sHA-CaV1.2-T1039Y and HA-CaV1.3a-T1033Y DHPR constructs. The locations of HA epitope, the position of DHPR mutation and carboxy-terminal PDZ domain-binding consensus are shown. (B) Hippocampal neurons were transfected with sHA-CaV1.2-T1039Y or HA-CaV1.3a-T1033Y plasmids with β3 and α2δ-1 auxiliary subunits as indicated. Seventy two hours after transfection neurons were stimulated for 30 s with 5 Hz EFS in the presence of 50 μm AP-5, 50 μm nifedipine and 20 μm bicuculline. Confocal images of the same field are shown for pCREB (green) and HA (red) staining as indicated. The pCREB nuclear staining of the same cells is shown at 40 × higher magnification. (C) An average increase in normalized pCREB nuclear staining (ΔpCREB) induced by 30-s 5-Hz EFS in hippocampal neurons transfected with sHA-CaV1.2-T1039Y (n = 42) or HA-CaV1.3a-T1033Y (n = 29). pCREB staining in neurons transfected with HA-CaV1.3a-T1033Y is significantly higher (***P < 0.05) than in neurons transfected with sHA-CaV1.2-T1039Y.