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. Author manuscript; available in PMC: 2013 Feb 22.
Published in final edited form as: J Am Chem Soc. 2012 Feb 9;134(7):3461–3471. doi: 10.1021/ja210126j

Figure 2.

Figure 2

Time course of visible absorption change (430 nm) following removal of O2 from oxyHb gels in the absence (filled symbols) or presence (open symbols) of IHP. Solid lines are fits to a double exponential with time constants indicated by dotted lines. Inset, Soret difference spectra obtained by subtracting the initial (t ≈ 0) deoxyHb spectrum from the deoxyHb spectra at 2 min (black), 30 min (red), 3 h (green), and 30 h (blue) after removal of O2.