AUUCU repeats form RNA aggregates and sequester hnRNP K in transgenic mouse brain.
A. In sagittal sections of 6 month-old transgenic mouse brains, RNA fluorescent in situ hybridization (FISH) showed distinct RNA aggregates (red) in neurons of the cortex, pontine nuclei and hippocampus. Similar sections of the brain of 6 month-old wild-type littermates showed DAPI stain of nuclei but no red FISH signal (not shown).
B. Co-localization of AUUCU-containing repeats (red) with hnRNPK (green) in the pontine nuclei of transgenic mice.
C. Co-localization of expanded AUUCU repeats (red) and hnRNP K (green) in the hippocampus of transgenic mice.