FIG. 1.

Acute intranasal instillation of cadmium chloride results in elevated olfactory bulb cadmium levels and reduced neurotransmitter release from the olfactory nerve for at least 28 days after exposure. (A) ICPMS revealed elevated levels of cadmium in the olfactory bulbs at every time point measured. This accumulation was not seen in cerebellum. (B) Ratio of the number of glomeruli receiving synaptic input from ORNs on the cadmium-exposed side compared with the vehicle-exposed side. The dashed line at one represents an equal number of responding glomeruli on each side. (C) Leftmost panel shows a sample baseline fluorescence image outlining the dorsal region of the olfactory bulbs viewable during in vivo imaging through a cranial window. Three following panels are pseudocolored response maps showing the increase in fluorescence in response to an odorant compared with preodor onset baseline. Callouts show the odorant-evoked response amplitude of the selected glomerulus during baseline and odorant presentation (denoted by horizontal black bars) portions of the trial. (D) Cumulative distributions of individual glomerular response amplitudes.