TABLE 1.

Adipose organ composition and parenchymal nerve fiber density in C57BL/6J obesity-prone mice

	Anterior Subcutaneous	Posterior Subcutaneous	Mediastinal	Mesenteric	Retroperitoneal	Abdominopelvic
C57BL/6J
No. unilocular adipocytes
28°C	56 ± 8###	14 ± 1	6 ± 3##	12 ± 1	2 ± 1	27 ± 2
6°C	25 ± 5*##	16 ± 3	0.6 ± 0.3	10 ± 1#	2 ± 0.2	15 ± 4*
No. UCP1-negative multilocular adipocytes
28°C	8 ± 0.3#	1 ± 0.2	0.2 ± 0.08#	0.7 ± 0.4	0	5 ± 0.8#
6°C	6 ± 1##	2 ± 0.4*	3 ± 0.1#	1 ± 0.3#	0.4 ± 0.1	11 ± 0.8
No. UCP1-positive multilocular adipocytes
28°C	12 ± 4###	0	8 ± 3	0	0	0.7 ± 0.4
6°C	54 ± 4 **###	0.6 ± 0.2##	7 ± 1	0.0 03 ± 0.002	0.001 ± 0.0002	7 ± 2*#
TH–positive fiber density
28°C	11 ± 2	2 ± 0.8	34 ± 0.7###	6 ± 2###	2 ± 1	16 ± 1
6°C	61 ± 5**#	6 ± 2##	50 ± 9	9 ± 2###	7 ± 2	31 ± 3*
SV129
No. unilocular adipocytes
28°C	16 ± 3	19 ± 0.2	0.1 ± 0.1	10 ± 0.8	2 ± 0.5	22 ± 1
6°C	9 ± 2*	16 ± 1*	0	4 ± 2**	2 ± 0.6	9 ± 1***
No. UCP1-negative multilocularadipocytes
28°C	2 ± 0.3	0.9 ± 0.5	0.05 ± 0.03	0	0.1 ± 0.1	9 ± 2
6°C	11 ± 3*	4 ± 1*	0.1 ± 0.04	10 ± 2**	0.1 ± 0	7 ± 1
No. UCP1-positive multilocularadipocytes
28°C	73 ± 6	0	5 ± 1	0	0.006 ± 0.006	15 ± 3
6°C	73 ± 5	6 ± 1***	9 ± 2	0.2 ± 0.03*	0.4 ± 0.1**	25 ± 3*
TH–positive fiber density
28°C	12 ± 7	1 ± 0.2	78 ± 2	1 ± 0.1	0.2 ± 0.1	16 ± 2
6°C	45 ± 8*	12 ± 2**	72 ± 26	20 ± 2**	2 ± 0.9	38 ± 0.8**

Data of obesity-resistant Sv129 mice from a previous work (26) are provided for comparison. Number of adipocytes × 10⁶. TH-positive fibers/100 adipocytes.

^*Indicates statistically significant difference between temperature conditions (same depot, same strain). *P < 0.05; **P < 0.01; ***P < 0.001.

^#Indicates statistically significant difference between strains (same depot, same temperature condition). ^#P < 0.05; ^##P < 0.01; ^###P < 0.001.Total number of adipocytes contained in the adipose organ of C57BL/6J and Sv129 results unchanged. P = 0.25 in 28°C mice; P = 0.18 in 6°C mice.