Fig. 8.
Analysis of HIV-1 infectivity. A: Western blot showing ABCA1 abundance in human monocyte-derived macrophages (MDM) and peripheral blood leukocytes (PBL) with or without activation with LXR agonist TO-901317 (1 μM). B: HIV-1 ADA collected from MDM and PBL was normalized by p24 content, and viral infectivity was tested on TZM-bl indicator cells by measuring luciferase activity. MDM were transfected with ABCA1-directed or control siRNA (see Materials and Methods). Results of one representative experiment out of three performed with cells from different donors are presented in relative luminescence units (RLU) as mean ± SEM of triplicate determinations. *P < 0.01 (versus PBL); #P < 0.01 (versus siRNAC).