TABLE 1.

Evaluation of the alkylating properties 5,6α-EC, 5,6β-EC, and SO

Compound	Solvent	Temperature	Nucleophile	Reaction
5,6α-EC	Ethanol	RT	—	NR
	Ethanol	RT	AE	NR
	Ethanol	RT	ME	NR
	Ethanol	RT	G	NR
	CM	RT	—	NR
	Ethanol	37°C	—	NR
	Ethanol	37°C	AE	NR
	Ethanol	37°C	ME	NR
	Ethanol	37°C	G	NR
	CM	37°C	—	NR
5,6β-EC	Ethanol	RT	—	NR
	Ethanol	RT	AE	NR
	Ethanol	RT	ME	NR
	Ethanol	RT	G	NR
	CM	RT	—	NR
	Ethanol	37°C	—	NR
	Ethanol	37°C	AE	NR
	Ethanol	37°C	ME	NR
	Ethanol	37°C	G	NR
	CM	37°C	—	NR
SO	Ethanol	RT	—	NR
	Ethanol	RT	AE	+
	Ethanol	RT	ME	+
	Ethanol	RT	G	+
	C.M.	RT	—	+
	Ethanol	37°C	—	NR
	Ethanol	37°C	AE	+
	Ethanol	37°C	ME	+
	Ethanol	37°C	G	+
	CM	37°C	—	+

Compounds were incubated at a concentration of 8 mg/ml for 48 h in the absence or presence of AE, ME, or G at a concentration 0.32 mol/l or in complete growth CM for eukaryote cells at the indicated temperatures. The reaction was followed as described in Materials and Methods.

+, reaction; AE, 2-aminoethanol; CM, culture medium; G, guanine; ME, 2-mercaptoethanol; NR, no reaction, RT, room temperature; SO, styrene oxide.