Fig. 3.

Inducible expression of apoA-IV decreases the rate of endogenous apoB100 secretion. A: Triplicate dishes of McA-RH7777 cells stably transfected with Tet-inducible apoA-IV were incubated without (−) or with (+) 1 µg/ml doxycycline (Dox) for 48 h. Cells were then radiolabeled with [³⁵S]Met and Cys for 4 h, followed by immunoprecipitation of cell lysates (C) and media (M) with anti-apoA-IV antibody, SDS-PAGE, and fluorography. B: Triplicate dishes of McA-RH7777 cells were incubated without or with Dox as indicated, and then subjected to a 10 min pulse with [³⁵S]Met and Cys followed by 0, 30, and 60 min chase with media containing cold amino acids. Data are mean ± SD (n = 3). Where error bars are not visible, the SD is less than the size of the symbol. Time points with different letters are P = 0.013 at 30 min and P = 0.034 at 60 min by ANOVA.