Fig. 5.

Effect of apoA-IV expression on VLDL size distribution. Tet-inducible apoA-IV McA-RH7777 cells were incubated without (−) or with (+) 1 µg/ml doxycycline (Dox) for 48 h and then treated with DMEM containing 20% FBS and 0.4 mM oleate complexed to 0.75% BSA for 4 h. A: Cell lysates (50 µg) were analyzed by 12.5% SDS-PAGE, followed by immunoblot analysis with anti-apoA-IV antibody. B: Media were harvested and subjected to cumulative rate flotation ultracentrifugation, as described in Refs. 12 and 36. The size distributions in the VLDL₁ fractions were determined using dynamic laser light scattering. C: Peak VLDL₁ particle diameters (mean ± SE; n = 3; *P < 0.001 by unpaired t-test).