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Impact of Dox-induced expression of apoA-IV on MTP mRNA and protein abundance. Tet-inducible apoA-IV McA-RH7777 cells were incubated in the absence (−) or presence (+) of 1 µg/ml doxycycline (Dox) for 48 h and apoA-IV (A) and MTP (B) gene expression were measured by qPCR, normalized to GAPDH, and shown as the fold increase in the presence of Dox. Data are mean ± SD; n = 3; *P < 0.001 by unpaired t-test. C: Immunoblot analysis of transfected human apoA-IV and endogenous rat MTP.
