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. 2012 Mar 5;196(5):573–587. doi: 10.1083/jcb.201110093

Figure 2.

Figure 2.

All 26S substrates are stabilized at the same critical concentration threshold of mutant N-htt expression. (A) All cytoplasmic UPS reporters accumulate above the same critical concentration threshold of mutant N-htt expression. Effect of N-htt(Q91)–chFP expression (x axis) on the fluorescence levels of different unstable GFP-degron constructs (y axis): Ub fusion degradation substrate UbG76V-GFP, Ub-independent substrate cODC-GFP, N-terminal end rule substrate Ub-R-GFP, and CL1-dependent substrate GFP-CL1. (B) ERAD substrates accumulate above the same critical concentration threshold of mutant N-htt expression. Relationship between N-htt(Q91)–chFP expression (x axis) plotted against the fluorescence levels of GFP-tagged ERAD substrates (y axis): GFP-ΔF508 and TCR-α–GFP. UbG76V-GFP was included for comparison. The data shown are from single representative experiments out of two independent repeats.